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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
09/04/2021 |
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Actualizado : |
09/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CERECETTO, V.; SMALLA , K.; NESME, J; GARAYCOCHEA, S.; FRESIA, P.; SØRENSEN, S.J.; BABIN, D.; LEONI, C. |
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Afiliación : |
MARÍA VICTORIA CERECETTO GONZÁLEZ, Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; KORNELIA SMALLA, Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany; JOSEPH NESME, University of Copenhagen, Department of Biology, Section of Microbiology, Copenhagen, Denmark; SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; SØREN JOHANNES SØRENSEN, University of Copenhagen, Department of Biology, Section of Microbiology, Copenhagen, Denmark; DOREEN BABIN, Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany; CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Reduced tillage, cover crops and organic amendments affect soil microbiota and improve soil health in Uruguayan vegetable farming systems. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
FEMS Microbiology Ecology, March 2021, Volume 97, Issue 3, fiab023. Doi: https://doi.org/10.1093/femsec/fiab023 |
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ISSN : |
0168-6496 (print); 1574-6941 (online) |
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DOI : |
10.1093/femsec/fiab023 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 05 August 2020; Accepted 04 February 2021; Published 06 February 2021.
Editor: Angela Sessitsch.
This work was supported by Instituto Nacional de Investigación Agropecuaria, Uruguay (Project INIA SA35 - Effect of agricultural management on soil microbiome-implication for plant growth and health), and by Julius Kuhn Institute, Germany. The work of Doreen Babin was supported by the German Federal Ministry of Education and Research (BMBF, Germany) in the framework of the project DiControl (http://dicontrol.igzev.de/de/; grant number 031B0514C) as part of the BonaRes initiative "Soil as a sustainable resource for the bioeconomy" (https://www.bonares.de/).
Corresponding author: Carolina Leoni, E-mail: cleoni@inia.org.uy |
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Contenido : |
ABSTRACT.
Conventional tillage and mineral fertilization (CTMF) jeopardize soil health in conventional vegetable production systems. Using a field experiment established in Uruguay in 2012, we aimed to compare the soil restoration potential of organic fertilization (compost and poultry manure) combined with conventional tillage and cover crop incorporated into the soil (CTOF) or with reduced tillage and the use of cover crop as mulch (RTOF). In 2017, table beet was cultivated under CTMF, CTOF and RTOF, and yields, soil aggregate composition and nutrients, as well as soil and table beet rhizosphere microbiota (here: bacteria and archaea) were evaluated. Microbiota was studied by high-throughput sequencing of 16S rRNA gene fragments amplified from total community DNA. RTOF exhibited higher soil aggregation, soil organic C, nutrient availability and microbial alpha-diversity than CTMF, and became more similar to an adjacent natural undisturbed site. The soil microbiota was strongly shaped by the fertilization source which was conveyed to the rhizosphere and resulted in differentially abundant taxa. However, 229 amplicon sequencing variants were found to form the core table beet rhizosphere microbiota shared among managements. In conclusion, our study shows that after only 5 years of implementation, RTOF improves soil health under intensive vegetable farming systems. Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. |
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Palabras claves : |
16S rRNA gene high-throughput amplicon sequencing; Fertilization; Rhizosphere; Soil properties; Soil restoration; Table beet; Tillage. |
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Asunto categoría : |
P30 Ciencia del suelo y manejo del suelo |
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Marc : |
LEADER 03272naa a2200325 a 4500
001 1061967
005 2021-04-09
008 2021 bl uuuu u00u1 u #d
022 $a0168-6496 (print); 1574-6941 (online)
024 7 $a10.1093/femsec/fiab023$2DOI
100 1 $aCERECETTO, V.
245 $aReduced tillage, cover crops and organic amendments affect soil microbiota and improve soil health in Uruguayan vegetable farming systems.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 05 August 2020; Accepted 04 February 2021; Published 06 February 2021. Editor: Angela Sessitsch. This work was supported by Instituto Nacional de Investigación Agropecuaria, Uruguay (Project INIA SA35 - Effect of agricultural management on soil microbiome-implication for plant growth and health), and by Julius Kuhn Institute, Germany. The work of Doreen Babin was supported by the German Federal Ministry of Education and Research (BMBF, Germany) in the framework of the project DiControl (http://dicontrol.igzev.de/de/; grant number 031B0514C) as part of the BonaRes initiative "Soil as a sustainable resource for the bioeconomy" (https://www.bonares.de/). Corresponding author: Carolina Leoni, E-mail: cleoni@inia.org.uy
520 $aABSTRACT. Conventional tillage and mineral fertilization (CTMF) jeopardize soil health in conventional vegetable production systems. Using a field experiment established in Uruguay in 2012, we aimed to compare the soil restoration potential of organic fertilization (compost and poultry manure) combined with conventional tillage and cover crop incorporated into the soil (CTOF) or with reduced tillage and the use of cover crop as mulch (RTOF). In 2017, table beet was cultivated under CTMF, CTOF and RTOF, and yields, soil aggregate composition and nutrients, as well as soil and table beet rhizosphere microbiota (here: bacteria and archaea) were evaluated. Microbiota was studied by high-throughput sequencing of 16S rRNA gene fragments amplified from total community DNA. RTOF exhibited higher soil aggregation, soil organic C, nutrient availability and microbial alpha-diversity than CTMF, and became more similar to an adjacent natural undisturbed site. The soil microbiota was strongly shaped by the fertilization source which was conveyed to the rhizosphere and resulted in differentially abundant taxa. However, 229 amplicon sequencing variants were found to form the core table beet rhizosphere microbiota shared among managements. In conclusion, our study shows that after only 5 years of implementation, RTOF improves soil health under intensive vegetable farming systems. Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.
653 $a16S rRNA gene high-throughput amplicon sequencing
653 $aFertilization
653 $aRhizosphere
653 $aSoil properties
653 $aSoil restoration
653 $aTable beet
653 $aTillage
700 1 $aSMALLA , K.
700 1 $aNESME, J
700 1 $aGARAYCOCHEA, S.
700 1 $aFRESIA, P.
700 1 $aSØRENSEN, S.J.
700 1 $aBABIN, D.
700 1 $aLEONI, C.
773 $tFEMS Microbiology Ecology, March 2021, Volume 97, Issue 3, fiab023. Doi: https://doi.org/10.1093/femsec/fiab023
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
24/07/2020 |
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Actualizado : |
27/01/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FÉLIX, M.L; ARMÚA-FERNÁNDEZ, M.T; PARODI, P.; BAZZANO , V.; MANGOLD, A.J.; VENZAL, J.M. |
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Afiliación : |
MARÍA L. FÉLIX, Laboratorio de Vectores Y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Salto, Universidad de La República, Rivera 1350, CP 50000, Salto, Uruguay.; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de La República, Rivera 1350, CP 50000, Salto, Uruguay.; PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VALENTIN BAZZANO, Laboratorio de Vectores Y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de La República, Rivera 1350, CP 50000, Salto, Uruguay.; ATILIO J. MANGOLD, Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, CC 22, CP 2300, Rafaela, Santa Fe, Argentina; JOSÉ M. VENZAL, Laboratorio de Vectores Y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de La República, Rivera 1350, CP 50000, Salto, Uruguay. |
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Título : |
Detection of a putative novel genotype of Anaplasma in gray-brocket deer (Mazama gouazoubira) from Uruguay. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Experimental and Applied Acarology, Vol. 81, number 4, pag. 575-583, Aug 2020. Doi: https://doi.org/10.1007/s10493-020-00523-0 |
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DOI : |
10.1007/s10493-020-00523-0 |
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Idioma : |
Inglés |
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Notas : |
Article histort: Received 13 December 2019/Accepted03 July 2020/Published09 July 2020.We would like to thank Comisión Sectorial de Investigación Científica (CSIC), Programa Iniciación a la Investigación 2017, Project ID 160 for the financial support to MLF. Corresponding author:Correspondence to José M. Venzal. |
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Contenido : |
Abstract:
Anaplasmataceae includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia, comprising a group of obligate intracellular bacteria. The genus Anaplasma has pathogenic species transmitted by ticks of veterinary and human health importance. Wild ungulates such as deer represent important reservoirs and amplifiers of Anaplasmataceae. The interaction between deer and domestic ruminants represents a serious problem due to the transmission of these pathogens through their ectoparasites. In the present study, we investigated the presence of Anaplasmataceae organisms in blood, tissues and tick samples of a gray-brocket deer (Mazama gouazoubira). The specimen was found dead in a farm in northeast Uruguay. PCRs targeting partial regions of 16S rRNA and groESL genes were carried out for Anaplasmataceae DNA detection. Moreover, several ectoparasites were identified: the chewing louse Tricholipeurus albimarginatus, the Neotropical deer louse fly Lipoptena mazamae, and the ticks Haemaphysalis juxtakochi and Rhipicephalus microplus. A consensus sequence of 1274 bp of 16S rRNA was generated for Anaplasma sp. from the M. gouazoubira blood sample. All ticks analysed by PCR assays were negative. No band was detected in any of the samples after PCR targeting groESL gene. Phylogenetic analysis using 16S rRNA partial gene sequences, clustered the putative novel genotype sequence obtained in this study, named Anaplasma sp. genotype Mazama?Uruguay, along with Anaplasma sp. detected in Mazama sp., Mazama americana and Mazama bororo, all deer species from Brazil. Furthermore, this cluster showed to be closely related to Anaplasma bovis sequences obtained from various ruminants and other mammals from several parts of the world. The pathogenicity as well as its infecting potential to other cervids or domestic ruminants is currently unknown. Further studies should be performed in order to characterize this novel species, especially targeting other genes. MenosAbstract:
Anaplasmataceae includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia, comprising a group of obligate intracellular bacteria. The genus Anaplasma has pathogenic species transmitted by ticks of veterinary and human health importance. Wild ungulates such as deer represent important reservoirs and amplifiers of Anaplasmataceae. The interaction between deer and domestic ruminants represents a serious problem due to the transmission of these pathogens through their ectoparasites. In the present study, we investigated the presence of Anaplasmataceae organisms in blood, tissues and tick samples of a gray-brocket deer (Mazama gouazoubira). The specimen was found dead in a farm in northeast Uruguay. PCRs targeting partial regions of 16S rRNA and groESL genes were carried out for Anaplasmataceae DNA detection. Moreover, several ectoparasites were identified: the chewing louse Tricholipeurus albimarginatus, the Neotropical deer louse fly Lipoptena mazamae, and the ticks Haemaphysalis juxtakochi and Rhipicephalus microplus. A consensus sequence of 1274 bp of 16S rRNA was generated for Anaplasma sp. from the M. gouazoubira blood sample. All ticks analysed by PCR assays were negative. No band was detected in any of the samples after PCR targeting groESL gene. Phylogenetic analysis using 16S rRNA partial gene sequences, clustered the putative novel genotype sequence obtained in this study, named Anaplasma sp. genotype Mazama?Uruguay, along with Anaplasma sp. detec... Presentar Todo |
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Palabras claves : |
ANAPLASMA SP; ANAPLASMATACEAE; CIERVOS; CIERVOS GRIS; MAZAMA GOUAZOUBIRA; PLATAFORMA DE SALUD ANIMAL; TICKS. |
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Thesagro : |
URUGUAY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03234naa a2200301 a 4500
001 1061238
005 2021-01-27
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s10493-020-00523-0$2DOI
100 1 $aFÉLIX, M.L
245 $aDetection of a putative novel genotype of Anaplasma in gray-brocket deer (Mazama gouazoubira) from Uruguay.$h[electronic resource]
260 $c2020
500 $aArticle histort: Received 13 December 2019/Accepted03 July 2020/Published09 July 2020.We would like to thank Comisión Sectorial de Investigación Científica (CSIC), Programa Iniciación a la Investigación 2017, Project ID 160 for the financial support to MLF. Corresponding author:Correspondence to José M. Venzal.
520 $aAbstract: Anaplasmataceae includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia, comprising a group of obligate intracellular bacteria. The genus Anaplasma has pathogenic species transmitted by ticks of veterinary and human health importance. Wild ungulates such as deer represent important reservoirs and amplifiers of Anaplasmataceae. The interaction between deer and domestic ruminants represents a serious problem due to the transmission of these pathogens through their ectoparasites. In the present study, we investigated the presence of Anaplasmataceae organisms in blood, tissues and tick samples of a gray-brocket deer (Mazama gouazoubira). The specimen was found dead in a farm in northeast Uruguay. PCRs targeting partial regions of 16S rRNA and groESL genes were carried out for Anaplasmataceae DNA detection. Moreover, several ectoparasites were identified: the chewing louse Tricholipeurus albimarginatus, the Neotropical deer louse fly Lipoptena mazamae, and the ticks Haemaphysalis juxtakochi and Rhipicephalus microplus. A consensus sequence of 1274 bp of 16S rRNA was generated for Anaplasma sp. from the M. gouazoubira blood sample. All ticks analysed by PCR assays were negative. No band was detected in any of the samples after PCR targeting groESL gene. Phylogenetic analysis using 16S rRNA partial gene sequences, clustered the putative novel genotype sequence obtained in this study, named Anaplasma sp. genotype Mazama?Uruguay, along with Anaplasma sp. detected in Mazama sp., Mazama americana and Mazama bororo, all deer species from Brazil. Furthermore, this cluster showed to be closely related to Anaplasma bovis sequences obtained from various ruminants and other mammals from several parts of the world. The pathogenicity as well as its infecting potential to other cervids or domestic ruminants is currently unknown. Further studies should be performed in order to characterize this novel species, especially targeting other genes.
650 $aURUGUAY
653 $aANAPLASMA SP
653 $aANAPLASMATACEAE
653 $aCIERVOS
653 $aCIERVOS GRIS
653 $aMAZAMA GOUAZOUBIRA
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTICKS
700 1 $aARMÚA-FERNÁNDEZ, M.T
700 1 $aPARODI, P.
700 1 $aBAZZANO , V.
700 1 $aMANGOLD, A.J.
700 1 $aVENZAL, J.M.
773 $tExperimental and Applied Acarology, Vol. 81, number 4, pag. 575-583, Aug 2020. Doi: https://doi.org/10.1007/s10493-020-00523-0
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